Human C5a (Complement Component 5a) CLIA Kit | G-EC-00357

Human C5a (Complement Component 5a) CLIA Kit | G-EC-00357 | Gentaur Clia Kits

Target Species: Human

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 9.38pg/mL

Detection Range: 15.63~1000pg/mL

UniProt ID:

Target Name: C5a

Target Synonym: C5a

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human C5a. You can calculate the concentration of Human C5a in the samples by comparing the RLU value of the samples to the standard curve.

Specificity: This kit recognizes Human C5a in samples. No significant cross-reactivity or interference between Human C5a and analogues was observed.

Application: This CLIA kit applies to the in vitro quantitative determination of Human C5a concentrations in Serum, plasma and other biological fluids.

Research Areas: Cell Biology, Metabolism, Immunology, Signal transduction

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Human C5a (Complement Component 5a) CLIA Kit DataSheet