QuicKey Pro Human Cortisol ELISA Kit | G-EC-06077
- 3 to 5 Working Days
QuicKey Pro Human Cortisol ELISA Kit | G-EC-06077 | Gentaur Elisa Kits
Target Species: Human
Assay Time: 1.5h
Detection Type: Colormetric
Detection Range: 6.25~400ng/mL
Target Name: Cortisol
Target Synonym: 11β, 17α, 21-Trihydroxypregn-4-ene-3, 20-dione
Tested Sample Types: Serum, Plasma, Urine, Saliva
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human Cortisol in samples. No significant cross-reactivity or interference between Human Cortisol and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human Cortisol concentrations in Serum, Plasma, Urine, Saliva.
Research Areas: Cell biology, Metabolism
Storage: An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.