Mouse GC (Glucagon) ELISA Kit | G-EC-00140

Mouse GC (Glucagon) ELISA Kit | G-EC-00140 | Gentaur Elisa Kits

Target Species: Mouse

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 18.75pg/mL

Detection Range: 31.25~2000pg/mL

UniProt ID: P55095

Target Name: GC

Target Synonym: GCG, GLP1, GLP2, GRPP

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse GC. During the reaction, Mouse GC in samples or Standard competes with a fixed amount of Mouse GC on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse GC. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Mouse GC in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Mouse GC in samples. No significant cross-reactivity or interference between Mouse GC and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Mouse GC concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Metabolism, Developmental biology, Signal transduction, Stem cells

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Mouse GC (Glucagon) ELISA Kit DataSheet

Citation #1