Mouse NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit | G-EC-01528

Mouse NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit | G-EC-01528 | Gentaur Clia Kits

Target Species: Mouse

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 37.5pg/mL

Detection Range: 62.5~4000pg/mL

UniProt ID:

Target Name: NOS2/iNOS

Target Synonym: NOS2A, HEP-NOS, I-NOS

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2/iNOS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NOS2/iNOS. You can calculate the concentration of Mouse NOS2/iNOS in the samples by comparing the RLU value of the samples to the standard curve.

Specificity: This kit recognizes Mouse NOS2/iNOS in samples. No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed.

Application: This CLIA kit applies to the in vitro quantitative determination of Mouse NOS2/iNOS concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Metabolism, Neuroscience

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Mouse NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit DataSheet