QuicKey Pro Human αFP (Alpha-Fetoprotein) ELISA Kit | G-EC-06111

QuicKey Pro Human αFP (Alpha-Fetoprotein) ELISA Kit | G-EC-06111 | Gentaur Elisa Kits

Target Species: Human

Type: Sandwich

Assay Time: 1.5h

Detection Type: Colormetric

Sensitivity: 0.47ng/mL

Detection Range: 0.78-50ng/mL

UniProt ID: P02771

Target Name: AFP

Target Synonym: AFP，Alpha-Fetoprotein

Tested Sample Types: Serum and plasma

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AFP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human AFP are added to the micro ELISA plate wells. Human AFP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human AFP. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human AFP. The concentration of Human AFP in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Human AFP in samples. No significant cross-reactivity or interference between Human AFP and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Human AFP concentrations in Serum and plasma .

Research Areas: Cancer, Cardiovascular, Developmental biology

Storage: An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.

QuicKey Pro Human αFP (Alpha-Fetoprotein) ELISA Kit DataSheet