Rat/Porcine E2 (Estradiol) ELISA Kit | G-EC-00060

Rat/Porcine E2 (Estradiol) ELISA Kit | G-EC-00060 | Gentaur Elisa Kits

Target Species: Rat/Porcine

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 1.00pg/mL

Detection Range: 1.56~100pg/mL

UniProt ID:

Target Name: E2

Target Synonym:

Tested Sample Types: Serum and plasma

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat/Porcine E2. During the reaction, Rat/Porcine E2 in samples or Standard competes with a fixed amount of Rat/Porcine E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat/Porcine E2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Rat/Porcine E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Rat/Porcine E2 in samples. No significant cross-reactivity or interference between Rat/Porcine E2 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Rat/Porcine E2 concentrations in Serum and plasma.

Research Areas: Signal transduction, Neuroscience, Developmental biology

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Rat/Porcine E2 (Estradiol) ELISA Kit DataSheet

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