ST/5-HT (Serotonin/5-Hydroxytryptamine) ELISA Kit | G-EC-00015

Gentaur Elisa

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$220.00 - $3,028.00

SKU:: G-EC-00015
UPC:
Availability:: 3 to 5 Working Days
Species:: Universal
Type:: Competitive
Duration:: 2.5h
Detection:: Colormetric
Sensitivity:: 9.38ng/mL
Range:: 15.63~1000ng/mL

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Description

ST/5-HT (Serotonin/5-Hydroxytryptamine) ELISA Kit | G-EC-00015 | Gentaur Elisa Kits

Target Species: Universal

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 9.38ng/mL

Detection Range: 15.63~1000ng/mL

UniProt ID:

Target Name: ST/5-HT

Target Synonym: 5HT, Enteramine, Thrombocytin, Thrombotonin, 3- (β-Aminoethyl) -5-hydroxyindole

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ST/5-HT. During the reaction, ST/5-HT in samples or Standard competes with a fixed amount of ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ST/5-HT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of ST/5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes ST/5-HT in samples. No significant cross-reactivity or interference between ST/5-HT and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of ST/5-HT concentrations in Serum, plasma and other biological fluids.

Research Areas: Neuroscience

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.