Dog E2 (Estradiol) ELISA Kit | G-EC-00061

Dog E2 (Estradiol) ELISA Kit | G-EC-00061 | Gentaur Elisa Kits

Target Species: Dog

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 1.88pg/mL

Detection Range: 3.12~200pg/mL

UniProt ID:

Target Name: E2

Target Synonym:

Tested Sample Types: Serum and plasma

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Dog E2. During the reaction, Dog E2 in samples or Standard competes with a fixed amount of Dog E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Dog E2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Dog E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Dog E2 in samples. No significant cross-reactivity or interference between Dog E2 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Dog E2 concentrations in Serum and plasma.

Research Areas: Signal transduction, Neuroscience, Developmental biology

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Dog E2 (Estradiol) ELISA Kit DataSheet