His-Tag Polyclonal Antibody | G-AB-05779

His-Tag Polyclonal Antibody | G-AB-05779 | Gentaur Antibodies

Overview: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

Category Type: Tag Antibody

Research Areas: Tags & Cell Markers

Synonyms: 6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, His tag, Polyhistidine Tag

Reactivity:

Host: Rabbit

Isotype: IgG

Gene ID:

Accession #:

Clonality: Polyclonal

Immunogen: 6X His synthetic peptide conjugated to KLH.

Clone #:

Conjugation: Unconjugated

Swissprot:

Santa Cruz:

Calculated MW:

Observed MW: 15 kDa

Concentration: 1 mg/mL

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Purification method: Affinity purification

Application: WB, ELISA

Dilution: WB 1:1000-1:3000, ELISA 1:20000

Storage: Store at -20°C. Avoid freeze / thaw cycles.

His-Tag Polyclonal Antibody DataSheet