Human/Monkey/Mouse E2 (Estradiol) ELISA Kit | G-EC-00058
- 3 to 5 Working Days
Human/Monkey/Mouse E2 (Estradiol) ELISA Kit | G-EC-00058 | Gentaur Elisa Kits
Target Species: Human/Monkey/Mouse
Assay Time: 2.5h
Detection Type: Colormetric
Detection Range: 9.37~600pg/mL
Target Name: E2
Tested Sample Types: Serum, Plasma, Urine, Saliva
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human/Monkey/Mouse E2. During the reaction, Human/Monkey/Mouse E2 in samples or Standard competes with a fixed amount of Human/Monkey/Mouse E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human/Monkey/Mouse E2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Human/Monkey/Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human/Monkey/Mouse E2 in samples. No significant cross-reactivity or interference between Human/Monkey/Mouse E2 and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human/Monkey/Mouse E2 concentrations in Serum, Plasma, Urine, Saliva.
Research Areas: Signal transduction, Neuroscience, Developmental biology
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.