His-Tag Monoclonal Antibody | G-AB-05825

His-Tag Monoclonal Antibody | G-AB-05825 | Gentaur Antibodies

Overview: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

Category Type: Tag Antibody

Research Areas: Tags & Cell Markers

Synonyms: 6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, His tag, Polyhistidine Tag

Reactivity:

Host: Mouse

Isotype: IgG

Gene ID:

Accession #:

Clonality: Monoclonal

Immunogen: Synthetic Peptide

Clone #: Clone:4F3

Conjugation: Unconjugated

Swissprot:

Santa Cruz:

Calculated MW:

Observed MW:

Concentration: 1 mg/mL

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification method: Protein A purification

Application: WB

Dilution: WB 1:3000-5000

Storage: Store at -20°C. Avoid freeze / thaw cycles.

His-Tag Monoclonal Antibody DataSheet