SARS-CoV-2 Neutralization Antibody ELISA Kit | G-EC-00072

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SARS-CoV-2 Neutralization Antibody ELISA Kit | G-EC-00072 | Gentaur Elisa Kits

Target Species: Human

Type: Competitive

Assay Time: 1.5h

Detection Type: Colormetric

Sensitivity: 9.38ng/mL

Detection Range: 15.63~500ng/mL

UniProt ID:

Target Name: SARS-CoV-2 Neutralization Antibody

Target Synonym: COVID 19 Neutralization Antibody, Anti-RBD, RBD Antibody, S1RBD Antibody

Tested Sample Types: Serum and plasma

Test Principle: This ELISA kit uses Competitive-ELISA as the method to semi-quantitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample. The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or standards/controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD) . After 37℃ incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SARS-CoV-2 Neutralization Antibody in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Human SARS-CoV-2 Neutralization Antibody in samples. No significant cross-reactivity or interference between Human SARS-CoV-2 Neutralization Antibody and analogues was observed.

Application: This ELISA kit applies to the in vitro semi-quantitative determination of Neutralization antibodies against SARS-CoV-2 in human serum or plasma.

Research Areas: SARS-CoV-2, COVID 19

Storage: An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.

SARS-CoV-2 Neutralization Antibody ELISA Kit DataSheet